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Alternatively, the stock society organisms could be grown in an acceptable liquid medium (i.e., Soybean–Casein Digest Broth or Sabouraud Dextrose Broth) as well as cells harvested by centrifugation, then washed and resuspended in sterile saline TS to obtain a microbial count of about one × 108 cfu for every mL. [Be aware—The estimate of
Just after completion of incubation, report the details of observation in Report and determine The share of recovery for new ton with Look at to Earlier approved media. If any new media obtaining for The very first time, then inoculated microorganism shall be taken for calculation.To prevent the unauthorized contamination, Ensure that all particula
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